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hal.structure.identifierUniversity of Tokyo
dc.contributor.authorPauty, Joris
hal.structure.identifierUniversity of Tokyo
dc.contributor.authorUsuba, Ryo
hal.structure.identifierautre
dc.contributor.authorCheng, Irene Gayi
hal.structure.identifierEcole Normale Supérieure - Département de Chimie
dc.contributor.authorHespel, Louise
HAL ID: 177640
ORCID: 0000-0002-5598-7194
hal.structure.identifierUniversity of Tokyo
dc.contributor.authorTakahashi, Haruko
hal.structure.identifierautre
dc.contributor.authorKato, Keisuke
hal.structure.identifierautre
dc.contributor.authorKobayashi, Masayoshi
hal.structure.identifierautre
dc.contributor.authorNakajima, Hiroyuki
hal.structure.identifierUniversity of Tokyo
dc.contributor.authorLee, Eujin
hal.structure.identifierLaboratoire d'analyse et modélisation de systèmes pour l'aide à la décision [LAMSADE]
dc.contributor.authorYger, Florian
HAL ID: 17768
ORCID: 0000-0002-7182-8062
hal.structure.identifierInstitut de biologie de Lille - UMS 3702 [IBL]
dc.contributor.authorSoncin, Fabrice
hal.structure.identifierUniversity of Tokyo
dc.contributor.authorMatsunaga, Yukiko
dc.date.accessioned2018-02-23T13:52:10Z
dc.date.available2018-02-23T13:52:10Z
dc.date.issued2018
dc.identifier.issn2352-3964
dc.identifier.urihttps://basepub.dauphine.fr/handle/123456789/17483
dc.language.isoenen
dc.subjectAngiogenesis inhibitorsen
dc.subjectDLL4en
dc.subjectHuman umbilical vein endothelial cellen
dc.subjectIn vitro 3D modelen
dc.subjectMicrovesselen
dc.subjectNotchen
dc.subjectSorafeniben
dc.subjectSprouting angiogenesisen
dc.subjectSunitiniben
dc.subjectVascular endothelial growth factoren
dc.titleA Vascular Endothelial Growth Factor-Dependent Sprouting Angiogenesis Assay Based on an In Vitro Human Blood Vessel Model for the Study of Anti-Angiogenic Drugsen
dc.typeArticle accepté pour publication ou publié
dc.description.abstractenAngiogenesis is the formation of new capillaries from pre-existing blood vessels and participates in proper vasculature development. In pathological conditions such as cancer, abnormal angiogenesis takes place. Angiogenesis is primarily carried out by endothelial cells, the innermost layer of blood vessels. The vascular endothelial growth factor-A (VEGF-A) and its receptor-2 (VEGFR-2) trigger most of the mechanisms activating and regulating angiogenesis, and have been the targets for the development of drugs. However, most experimental assays assessing angiogenesis rely on animal models. We report an in vitro model using a microvessel-on-a-chip. It mimics an effective endothelial sprouting angiogenesis event triggered from an initial microvessel using a single angiogenic factor, VEGF-A. The angiogenic sprouting in this model is depends on the Notch signaling, as observed in vivo. This model enables the study of anti-angiogenic drugs which target a specific factor/receptor pathway, as demonstrated by the use of the clinically approved sorafenib and sunitinib for targeting the VEGF-A/VEGFR-2 pathway. Furthermore, this model allows testing simultaneously angiogenesis and permeability. It demonstrates that sorafenib impairs the endothelial barrier function, while sunitinib does not. Such in vitro human model provides a significant complimentary approach to animal models for the development of effective therapies.en
dc.relation.isversionofjnlnameEBioMedicine
dc.relation.isversionofjnlvol27en
dc.relation.isversionofjnldate2018-01
dc.relation.isversionofjnlpages225-236en
dc.relation.isversionofdoi10.1016/j.ebiom.2017.12.014en
dc.contributor.countryeditoruniversityotherJAPAN
dc.contributor.countryeditoruniversityotherFRANCE
dc.subject.ddclabelMaladiesen
dc.relation.forthcomingnonen
dc.relation.forthcomingprintnonen
dc.description.ssrncandidatenonen
dc.description.halcandidateouien
dc.description.readershiprechercheen
dc.description.audienceInternationalen
dc.relation.Isversionofjnlpeerreviewedouien
dc.relation.Isversionofjnlpeerreviewedouien
dc.date.updated2018-02-23T13:24:30Z
hal.identifierhal-01716252*
hal.version1*
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