Transcribed-ultra conserved region expression is associated with outcome in high-risk neuroblastoma

Scaruffi, Paola; Stigliani, Sara; Moretti, Stefano; Coco, Simona; Valdora, Francesca; Garaventa, Alberto; Bonassi, Stefano; Tonini, Gian Paolo

Scaruffi, Paola; Stigliani, Sara; Moretti, Stefano; Coco, Simona; Valdora, Francesca; Garaventa, Alberto; Bonassi, Stefano; Tonini, Gian Paolo (2009), Transcribed-ultra conserved region expression is associated with outcome in high-risk neuroblastoma, BMC Cancer, 9, 441. http://dx.doi.org/10.1186/1471-2407-9-441

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Type

Article accepté pour publication ou publié

Date

2009

Journal name

BMC Cancer

Volume

Number

441

Publisher

BioMed Central

Abstract (EN)

Background: Neuroblastoma is the most common, pediatric, extra-cranial, malignant solid tumor. Despite multimodal therapeutic protocols, outcome for children with a high-risk clinical phenotype remains poor, with long-term survival still less than 40%. Hereby, we evaluated the potential of non-coding RNA expression to predict outcome in high-risk, stage 4 neuroblastoma. Methods: We analyzed expression of 481 Ultra Conserved Regions (UCRs) by reverse transcription-quantitative real-time PCR and of 723 microRNAs by microarrays in 34 high-risk, stage 4 neuroblastoma patients. Results: First, the comparison of 8 short- versus 12 long-term survivors showed that 54 UCRs were significantly (P < 0.0491) over-expressed in the former group. For 48 Ultra Conserved Region (UCRs) the expression levels above the cut-off values defined by ROC curves were strongly associated with good-outcome (OS: 0.0001

Subjects / Keywords

ultra-conserved regions; non-coding RNA expression; neuroblastoma