Thumbnail - Request a copy

Gene Expression Profiling Identities Eleven DNA Repair Genes Down Regulated During Mouse Neural Crest Cell Migration

Coco, Simona; Truini, Mauro; Mirisola, Valentina; Falugi, Carla; Di Candia, Michele; Scaruffi, Paola; Moretti, Stefano; Brizzolara, Antonella; Albino, Domenico; Tonini, Gian Paolo (2011), Gene Expression Profiling Identities Eleven DNA Repair Genes Down Regulated During Mouse Neural Crest Cell Migration, The International Journal of Developmental Biology, 55, 1, p. 65-72. http://dx.doi.org/10.1387/ijdb.092970da

Type
Article accepté pour publication ou publié
Date
2011
Journal name
The International Journal of Developmental Biology
Volume
55
Number
1
Publisher
UBC Press
Pages
65-72
Publication identifier
Metadata
Show full item record
Author(s)
Coco, Simona
Truini, Mauro
Mirisola, Valentina
Falugi, Carla
Di Candia, Michele
Scaruffi, Paola
Moretti, Stefano cc
Brizzolara, Antonella
Albino, Domenico
Tonini, Gian Paolo
Abstract (EN)
Neural Crest Cells (NCCs) are a transient multipotent migratory cells that derive from embryonic neural crest structure and in turn neural crest derives from the margin of the neural tube. DNA repair genes have been shown to be expressed in the early stages of mammalian development probably to reduce possible replication errors and genotoxic damages. Several birth defects and some cancers are due to inappropriate or defective DNA repair machinery indicating that a right activity of DNA repair genes in the early stages of fetal development is essential for an appropriate DNA function. A complete profile of DNA repair genes during embryo development has not been yet assess. We performed a genome-wide gene expression analysis by high-density oligo-microarray of mouse NCCs at E8.5, at E13.3 and at P90 and we found 11 genes involved in DNA repair activity overexpressed in the early stages of mouse embryo development whereas their expression become undetectable in the adrenal medulla of adult mouse. Among the 11 genes, 6 were never been observed high expressed in mouse embryology. Our results suggest that high expression of NCCs DNA repair genes in early embryonal stages needs to maintain DNA integrity. Failure of some of these genes may be associated to both genetic and cancer diseases.
Subjects / Keywords
DNA Repair Genes; Neural Crest Cells